Clinical effect of Zhanjinhuoxue Formula combined tower-type pad natural traction for treating pure thoracolumbar compression fracture / 重庆医学

Objective To investigate the clinical effect of Zhanjinhuoxue Formula combined with the tower-type pad natural traction for the treatment of pure thoracolumbar compression fracture.Methods Fifty patients with thoracolumbar compression fractures treated in our hospital from January to December 2014 were selected and divided into the observation group(n=25) and control group(n=25).The control group was given the tower-type natural traction method,while the observation group was given Zhanjinhuoxue Formula combined with the tower-type pad natural traction method.The curative effect,pain score,activity ability score,analgesic drugs score,bone mineral density (BMD) and the Japanese Orthopedic Association(JOA) score were compared between the two groups.Results The effective rate was 92.00％ in the observation group and 68.00％ in the control group,the difference between the two groups was statistically significant (x2 =4.50,P＜0.05).The pain score,activity ability score and analgesic drugs score after 6-month treatment in the observation group were significantly lower than those in the control group (P＜0.05);the BMD and JOA scores in the observation group were significantly higher than those in the control group (P＜0.05).Condusion Zhanjinhuoxue Formula coordinated by the tower-type pad natural traction method can conduce to alleviate the pain symptom in the patients with pure thoracolumbar compression fracture,increases the movement function and improves the treatment effect.

Inhibition of SIRT6 in prostate cancer reduces cell viability and increases sensitivity to chemotherapeutics

SIRT6 is an important histone modifying protein that regulates DNA repair, telomere maintenance, energy metabolism, and target gene expression. Recently SIRT6 has been identified as a tumor suppressor and is down-regulated in certain cancer types, but not in other cancers. From deposited gene profiling studies we found that SIRT6 was overexpressed in prostate tumors, compared with normal or paratumor prostate tissues. Tissue micro-array studies confirmed the higher levels of SIRT6 in both prostate tumor tissues and prostate cancer cells than in their normal counterparts. Knockdown of SIRT6 in human prostate cancer cells led to sub-G1 phase arrest of cell cycle, increased apoptosis, elevated DNA damage level and decrease in BCL2 gene expression. Moreover, SIRT6-deficiency reduced cell viability and enhanced chemotherapeutics sensitivity. Taken together, this study provides the first evidence of SIRT6 overexpression in human prostate cancer, and SIRT6 regulation could be exploited for prostate cancer therapy.

Effect of occupational manganese exposure on uric Acid levels in human urine / 生物医学与环境科学（英文）

<p><b>OBJECTIVE</b>To investigate the effect of long-term and low-level occupational Mn exposure on the level of uric acid (UA) in human urine.</p><p><b>METHODS</b>In this study, 65 volunteers were recruited, who were working on welding and foundry work in an plant in Gansu province, China. Additionally, 29 control samples were collected from individuals who did not have any history of excessive Mn exposure. An improved high performance liquid chromatography system equipped with a diode-array detector (HPLC-DAD) method was developed to determine the UA level in human urine. A Spectra AA 220 Atomic Absorption Spectrophotometer (AAS) was used to measure the Mn level in the urine.</p><p><b>RESULTS</b>The analytical method was validated for concentrations ranging from 3.82-45.84 μg/mL with acceptable accuracy, precision, and recovery. Overall, the UA levels of Mn exposure samples were significantly lower than that of control samples (P<0.05).</p><p><b>CONCLUSION</b>The practical method developed here is suitable for both routine monitoring of UA level in human urine and metabolism research. Long-term and low-level occupational Mn exposure may lead to a lower UA level in urine, and UA might be an indicator of the early stage of manganism.</p>

Study on extraction process of Scutellaria barbata / 中成药

Objective:To study the optimum ethanol extraction process. Methods: The extraction rate of extractum and total flavonoids as well scutellarin was choosen as the assessment index. The optimum ethanol extraction process was selected with the orthogonal design. Results: The optimum ethanol extraction process conditions follow as: adding 10 fold of 75% ethanol, extracting for 2.0h and twice in all. Conclusion: The average extraction rate of extractum and total flavonoids as well scutellarin was 13.6%, 91.7%, 78.4%(n=3), respectively.